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. 2020 Apr 17;8:240. doi: 10.3389/fchem.2020.00240

Figure 3.

Figure 3

One-step enrichment of glycopeptides from WT and FUT8 KO CHO-K1 cells. (A) Distribution of IGPs, glycosites, glycans, and glycoproteins identified from WT and FUT8 KO CHO-K1 cells. (B) Glycosite frequency on the identified glycoproteins. (C) N-glycosylation of laminin subunit alpha-5 with 10 glycosites, including the structure type and relative abundance (peptide spectrum match) of glycans on each glycosite. (D) Heat map of glycosites, intact glycopeptides (IGPs), core-fucosyalted IGPs, and glycans of WT and FUT8 KO CHO-K1 cells.