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. 2020 Apr 17;11:513. doi: 10.3389/fmicb.2020.00513

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Copyright © 2020 Wang, Liu, Mo, Huo, Wang, Zhong, Jia, Zhang, Chen, Chen and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immuno-blockade of NcHig enhanced the infection of RYSV in the CNS of N. cincticeps. RT-qPCR assay of viral genome copies of RYSV in the head (A) and alimentary canal (B) of N. cincticeps at 6 days post-microinjection of NcHig antibody or pre-immune antibody as the control. The RYSV genome copies in the individual samples (n = 27) from viruliferous N. cincticeps were calculated as the log of the copies number/μg tissue RNA based on the standard curve for the RYSV N gene. The analysis was based on three biological repeats. P-values were estimated using a Student’s t-test. (C) RYSV infection of the CNS of viruliferous N. cincticeps at 6 days post-microinjection of NcHig antibody or pre-immune antibody, as analyzed by an immunofluorescence assay. Virus-infected heads were immunolabeled with RYSV-M-rhodamine (red) and α-tubulin-FITC (green). ag, abdominal ganglion; br, brain; sg, salivary gland. Bars, 50 μm.