Fluorescent in situ hybridization (FISH) of Mycobacterium leprae in skin biopsy specimens collected from patients suspected of having cutaneous leprosy in Burkina Faso. Rows 1, 2, and 3 show positive skin biopsy specimens from patients P2, P4, and P5, respectively (Table 1); row 4 shows a negative-control skin biopsy specimen from patient 8111270308 (Table 1). (A) Ziehl-Neelsen staining; (B) FISH-DAPI staining; (C) results of the GenoType LepraeDR test and PCR amplification of the rpoB gene. +, positive; −, negative. Bar, 10 μm.