TABLE 2.
In silico and experimental tests of the specificity of the Alexa Fluor 555-labeled fluorescent oligonucleotide probea
| Test and bacterium | GenBank accession no. or source | In silico hybridization (%) or exptl hybridization resultb |
|---|---|---|
| In silico | ||
| Mycobacterium leprae | CP029543.1 | 100 |
| Mycobacterium lepromatosis | EU203594.2 | 85 |
| Mycobacterium haemophilum | CP011883.2 | 65 |
| Mycobacterium tuberculosis | CP023640.1 | 65 |
| Mycobacterium bovis BCG | CP033311.1 | 65 |
| Mycobacterium ulcerans | LR135168.1 | 60 |
| Mycobacterium marinum | CP024190.1 | 60 |
| Mycobacterium abscessus | CP022234.1 | 60 |
| Mycobacterium fortuitum | CP014258.1 | 65 |
| Staphylococcus aureus | LR134267.1 | 65 |
| Streptococcus pyogenes | AY583221.1 | 65 |
| Pseudomonas aeruginosa | LR130534.1 | 60 |
| Total | 12 | |
| Experimental specificity | ||
| Mycobacterium ulcerans | Our laboratory | ND |
| Mycobacterium marinum | Our laboratory | ND |
| Mycobacterium tuberculosis complex | Our laboratory | ND |
| Staphylococcus aureus | Our laboratory | ND |
| Streptococcus pyogenes | Our laboratory | ND |
| Pseudomonas aeruginosa | Our laboratory | ND |
| Klebsiella pneumoniae | Our laboratory | ND |
| Total | 7 |
a
5′-CAACTCCTCAGGCAAGTTGA-3′.
b
ND, hybridization was not detected by fluorescence microscopy.