TABLE 3.
Results of the laboratory investigation of skin samplesa
| Patient code | Sample | Result of: |
GenoType LepraeDR result |
|||||
|---|---|---|---|---|---|---|---|---|
| Ziehl-Neelsen microscopy (BI) | Fluorescent microscopy | PCR for rpoB | Detection of M. leprae | Rifampin resistance | Dapsone resistance | Ofloxacin resistance | ||
| P1 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P2 | Skin biopsy | Pos (4+) | Pos | ML | ML | Sensitive | Sensitive | Sensitive |
| P3 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P4 | Skin biopsy | Pos (3+) | Pos | ML | ML | Sensitive | Sensitive | Sensitive |
| P5 | Skin biopsy | Pos (1+) | Pos | Neg | Neg | NA | NA | NA |
| P6 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P7 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P8 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P9 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P10 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P11 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P12 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P13 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P14 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| P15 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P16 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P17 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P18 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P19 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P20 | Slit skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P21 | Skin biopsy | Pos (5+) | Pos | ML | NT | NT | NT | NT |
| Skin swab (3) | Pos (3+) | Pos | ML | NT | NT | NT | NT | |
| P22 | Swab of ulcerative skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P23 | Swab of ulcerative skin | Neg | Neg | Neg | NA | NA | NA | NA |
| P24 | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| 8111270308* | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| 8121367852* | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
| 9011404509* | Skin biopsy | Neg | Neg | Neg | NA | NA | NA | NA |
a
NA, not applicable; Neg, negative; Pos, positive; *, negative-control sample collected from patient admitted to the Institut Hospitalier Universitaire Méditerranée Infection, Marseille, France, and presenting skin lesions devoid of any evidence of mycobacteria; NT, not tested; 3, three swabs collected from three types of skin lesions (skin wound, scaly skin evoking cutaneous leishmaniasis, and skin papules with raised extremities) in patient P21; BI, bacterial index determined using Ridley’s logarithmic scale (22).