TABLE 2.
Species | SOC positive/BCID-FP negative | PCR/sequencing | Interpretation | SOC negative/BCID-FP positive | PCR/sequencing | Interpretation |
---|---|---|---|---|---|---|
C. albicans | ||||||
Clinical sample (Retrospective) | 1 | Positive for C. albicans | False negative | |||
Contrived sample | 1a | ND | False negative | 1b | ND | False positive |
C. glabrata | ||||||
Clinical sample (Retrospective) | 1c | Negative for C. glabrata | Indeterminate | 2d | Positive for C. glabrata | True positive |
C. guillermondii | ||||||
Contrived sample | 1e | ND | False negative | |||
C. kefyr | ||||||
Contrived sample | 2f | ND | False positive | |||
C. lusitaniae | ||||||
Clinical sample (Retrospective) | 1 | Positive for C. lusitaniae | False negative | |||
Contrived sample | 1g | ND | False positive | |||
C. parapsilosis | ||||||
Clinical sample (Retrospective) | 1h | Positive for C. parapsilosis | False negative | 1i | Positive for C. parapsilosis | True positive |
C. tropicalis | ||||||
Clinical sample (Retrospective) | 1j | Positive for C. tropicalis | True positive | |||
Fusarium spp. | ||||||
Contrived sample | 1k | ND | False negative | |||
Rhodotorula spp. | ||||||
Contrived sample | 2l | ND | False negative | 1m | ND | False positive |
Total | 9 | 9 |
The sample was spiked with C. albicans ATCC10231. It was flagged positive on day 6 but was negative by the BCID-FP panel.
The sample was spiked with C. dubliniensis ATCCMYA-578. C. dubliniensis was correctly detected by the BCID-FP panel, but the sample was also positive for C. albicans and C. kefyr (same sample discussed in footnote f).
The sample grew C. albicans, C. glabrata, and C. dubliniensis. The BCID-FP panel detected C. albicans, C. dubliniensis, but not C. glabrata. Subsequently, C. glabrata was not detected in the residual of that sample by PCR/sequencing. This sample is also listed as Case 1 in Table 3.
These two samples are also listed as Case 6 and 7 in Table 3.
The sample was spiked with C. guilliermondii ATCC90198. It was flagged positive on day 2 but was negative by the BCID-FP panel.
One sample was spiked with C. dubliniensis ATCCMYA-578. C. dubliniensis was correctly detected by the BCID-FP panel, but the sample was also positive for C. kefyr and C. albicans (same sample discussed in footnote b). The other sample was spiked with C. auris CDC number 0390. C. auris was correctly detected by the BCID-FP panel, but the sample was also positive for C. kefyr.
The sample was spiked with C. neoformans ATCC14116. C. neoformans was correctly detected by the BCID-FP panel, but the sample was also positive for C. lusitaniae.
This sample is also listed as Case 3 in Table 3.
This sample is also listed as Case 4 in Table 3.
This sample is also listed as Case 5 in Table 3.
The sample was spiked with Fusarium dimerum CBS110317. It was flagged positive on day 3 but was negative by BCID-FP Panel.
Two contrived samples were each spiked with Rhodotorula mucilaginosa ATCC66034 and R. mucilaginosa ATCC9449 and flagged positive on day 3 and day 6, respectively, but were negative by BCID-FP Panel.
The sample was spiked with C. auris CDCnumber 0389. C. auris was correctly detected by BCID-FP Panel, but the sample was also positive for Rhodotorula.
ND, not done.