Table 1.

Primers and probes for qPCR

Gene	Primer	Sequence
ε‐globin	Forward	5′‐ACA ACC TCA AGC CCG C‐3′
	Reverse	5′‐AGA CAC CAG CTT CTG CC‐3′
	Probe	5′‐HEX‐TGC CAA AGT GAG TAG CCA GAA TAA TC‐ZEN‐IBFQ‐3′
γ‐globin	Forward	5′‐ACC TGG ATG ATC TCA AGG G‐3′
	Reverse	5′‐CAG TCA CCA TCT TCT GCC‐3′
	Probe	5′‐Cy5‐TGC CGA AAT GGA TTG CCA AAA CGG TC‐IBRQ‐3′
β‐globin	Forward	5′‐ ACA ACC TCA AGG GCA CC‐3′
	Reverse	5′‐ACA CCA GCC ACC ACT TTC‐3′
	Probe	5′‐FAM‐ TGC CAA AGT GAT GGG CCA GCA CAC AG‐IBRQ‐3′
α‐globin	Forward	5′‐TCC CCA CCA CCA AGA CCT AC‐3′
	Reverse	5′‐CCT TAA CCT GGG CAG AGC C‐3′
	Probe	5′‐HEX‐TCC CGC ACT TCG ACC TGA GCC A‐IBRQ‐3