Figure 5.

NaB suppresses APP expression and increases the BDNF/NEP level in a GPR109A-dependent manner in the protein level. The N2a cells were pretreated for 1 hour with 1 mM PTX and then incubated for 24 hours with 2 mM NaB. (a) Protein expression of APP, NEP, BDNF, and GPR109A in N2a cells was assessed by western blotting (n = 3); β-tubulin was used as an internal loading control. (b–e) Quantitative analysis of the expression of APP, BDNF, NEP, and GPR109A of no treatment (NT), NaB-treated (NaB), and PTX and NaB-treated (PTX+NaB) (n = 3, means ± SD, Student's t-test, ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001). 2 mM NaB reduced expression of APP and increased expression of BDNF and NEP. Simultaneously, NaB promoted the expression of the GPR109A.