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. 2020 Mar 17;11(5):1297–1308. doi: 10.1111/1759-7714.13397

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Circ_0038467 acted as a sponge of miR‐338‐3p in 16HBE cells. (a) Schematic model of the miR‐338‐3p‐binding sites within circ_0038467 identified by the online database Circinteractome and the mutant in the target sequence. (b) Luciferase activity was monitored in 16HBE cells cotransfected with circ_0038467 wild‐type reporter construct (circ_0038467‐WT) or circ_0038467 mutant‐type construct (circ_0038467‐MUT) and miR‐338‐3p mimic or miR‐NC mimic. miR‐NC, miR‐338‐3p. (c) MiR‐338‐3p expression was detected by qRT‐PCR in 16HBE cells after LPS treatment. (d) MiR‐338‐3p expression was determined in 16HBE cells transfected with si‐NC or si‐circ#2. si‐circ#2: si‐circ_0038467#2. (e) Cell lysates were incubated with anti‐Ago2 or anti‐IgG antibody, and enrichment of circ_0038467 and miR‐338‐3p were evaluated by qRT‐PCR. *P < 0.05.

Inline graphic — Circ_0038467 acted as a sponge of miR‐338‐3p in 16HBE cells. (a) Schematic model of the miR‐338‐3p‐binding sites within circ_0038467 identified by the online database Circinteractome and the mutant in the target sequence. (b) Luciferase activity was monitored in 16HBE cells cotransfected with circ_0038467 wild‐type reporter construct (circ_0038467‐WT) or circ_0038467 mutant‐type construct (circ_0038467‐MUT) and miR‐338‐3p mimic or miR‐NC mimic. miR‐NC, miR‐338‐3p. (c) MiR‐338‐3p expression was detected by qRT‐PCR in 16HBE cells after LPS treatment. (d) MiR‐338‐3p expression was determined in 16HBE cells transfected with si‐NC or si‐circ#2. si‐circ#2: si‐circ_0038467#2. (e) Cell lysates were incubated with anti‐Ago2 or anti‐IgG antibody, and enrichment of circ_0038467 and miR‐338‐3p were evaluated by qRT‐PCR. *P < 0.05.