Table 4.
HSA Type | t °C | r1, mM−1s−1 | r2, mM−1s−1 | n * | τcor (ns) ** | r1, mM−1s−1 | r2, mM−1s−1 | r2/r1 |
---|---|---|---|---|---|---|---|---|
per albumin molecule | per nitroxide radical | |||||||
HSA-NIT-8 | 25 | 0.80 ±0.03 | 11.2 ±0.2 | 2.4 | 14/2.6 | 0.33 | 4.7 | 14.2 |
HSA-NIT-7 | 25 | 1.99 ±0.05 | 27.9 ±0.5 | 3.9 | 14/2.7 | 0.51 | 7.2 | 14.1 |
37 | 1.51 ±0.03 | 20.6 ±0.1 | 3.9 | 0.39 | 5.3 | 13.6 | ||
HSA-NIT-6 | 25 | 1.86 ±0.04 | 20.4 ±0.3 | 3.9 | 10/2.1 | 0.48 | 5.2 | 10.8 |
37 | 1.51 ±0.05 | 16.6 ±0.3 | 3.9 | 0.39 | 4.3 | 11.0 | ||
HSA-NIT-5 | 25 | 2.24 ±0.08 | 27.7 ±0.2 | 4.8 | 10/1.9 | 0.47 | 5.8 | 12.3 |
* Nitroxides per albumin molecule. ** «slow»/«fast» motional components at 25 °C.