Figure 7.

Effect of 8a on apoptosis-related protein expression. (A) and (B) HCT116 cells were treated with 20 μg/mL 8a for different time-periods. PARP, caspase 3, cleaved caspase 3, caspase 9, cleaved caspase 9, Bcl-2, phosphorylated Bcl-2, Bcl-XL, phosphorylated Bcl-xL and Bax were detected by western blot analysis; β-actin was used as a loading control in each lane. Representative data of three independent experiments are shown; (C) and (D) Grayscale analysis of the relative expression of apoptosis-related proteins detected by Western blotting. Data represent the mean ± standard deviation of three independent experiments. ** p < 0.01, * p < 0.05; compared with control group. (E) Cells were exposed to 8a (20 μg/mL) and/or zVAD-FMK (5 mM) for 24 h, and cell viability was measured by MTT assay. Data represent the mean ± standard deviation of three independent experiments * p < 0.05, ** p < 0.01.