. 2020 Apr 9;25(7):1721. doi: 10.3390/molecules25071721

Table 1.

Method validation data for the quantitative determination of the six tested compounds.

Parameters of Validation		Compound
Parameters of Validation		Lamalbid (1)		Chlorogenic acid (3)		Rutin (4)		Verbascoside (6)		Quercetin Malonylhexoside (7)		Tiliroside (8)
Linearity	Regression Equation	y = 5.4985 × 10⁶x + 5.2368 × 106		y = 1.4796 × 10⁷x − 11,665.0421		y = 9082.6404x + 11,626.4171		y = 8.0551 × 10⁶x − 45,113.1144		y = 6914.3069x − 79,129.6939		y = 16,857.3222x + 14,746.2045
	r	0.9751		0.9923		0.9964		0.998		0.9958		0.9853
	R²	0.9508		0.9847		0.9928		0.9961		0.9916		0.9709
	test F (α = 0.99)	444.19		1805.34		211.16		5825.75		2710.48		767.05
Recovery (n = 9)		(%)	CV (%)	(%)	CV (%)	(%)	CV (%)	(%)	CV (%)	(%)	CV (%)	(%)	CV (%)
	60% of content	118.79	1.71	109.88	9.08	103.53	1.42	96.19	2.79	108.03	0.21	108.44	7.08
	100% of content	115.55	4.12	96.49	7.20	96.57	9.25	140.52	9.53	109.02	5.82	90.12	8.92
	140% of content	111.17	2.22	110.51	4.14	98.33	4.93	101.94	2.26	97.48	5.07	97.73	2.24
Repeatability (n = 6)	mean (ng)	2117.47		871.99		139.95		474.20		308.65		111.53
	S (ng)	70.16		115.14		23.46		13.73		13.37		8.97
	CV (%)	3.30		13.20		16.76		2.90		4.30		8.04
	$\tilde{x} \pm Δ x$ (α = 0.05) (ng)	2117.47 ± 50.00		871.99 ± 115.02		139.95 ± 23.44		474.20 ± 13.72		308.65 ± 13.36		111.53 ± 8.96
Intermediate Precision (n = 6)	mean (ng)	2131.45		971.62		141.04		474.20		321.50		109.41
	S (ng)	50.00		133.02		13.52		10.64		16.67		7.89
	CV (%)	2.35		13.69		9.59		2.24		5.19		7.21
	$\tilde{x} \pm Δ x$ (α = 0.05) (ng)	2131.45 ± 20.00		971.62 ± 65.87		141.04 ± 6.69		474.20 ± 6.14		321.50 ± 8.25		109.41 ± 7.88
LOQ ¹ (ng) (n = 6)		1950		264		55		140		214		51
LOD ² (ng) (n = 6)		640		87		18		46		70		17
Range (ng)		1950–3400		264–1300		55–600		140–1500		214–800		51–200

CV—coefficient of variation; S—standard deviation; ¹ LOQ—limit of quantification (per injection); ² LOD—limit of detection (per injection).