Table 1.
Antibody and recombinant protein sources and conditions used.
| Supplier | Cat# | Isotype | Epitope | Working dilution | Comments | |
|---|---|---|---|---|---|---|
| Antibodies | ||||||
| TSPO1 (18 kDa, 169aa) | Abcam | ab109497 | rMonoAb (EPR5384) | aa150-169 | 1:100K | Good reactivity & selectivity for 18 kDa TSPO |
| ThermoFisher | MA5-24844 | rMonoAb (4H2) | C-terminal | 1:30K | Good reactivity & selectivity for 18 kDa TSPO | |
| ThermoFisher | PA5-75544 | rPolyAb | 124-169 | 1:10K | Some cross reactivity at higher MW | |
| Bio-Rad | VPA-00789 | rPolyAb | C-terminal | 1:2K | Low reactivity & selectivity for human brain TSPO | |
| Origene | TA303138 | gPolyAb | aa156-169 | NA | Major band at 35 kDa? | |
| Abnova | H00000706-M01 | Mouse IgG1k (3D8-B2) | Unknown | NA | No major band identified | |
| Santa Cruz Biotech | Sc-20120 | rPolyAb (FL-169) | Unknown | NA | No major band identified | |
| GFAP (50,48,45,and 43 kDa) | Chemicon/Millipore | MAB360 | Mouse IgG1 | Unknown | 1:100K | |
| Vimentin (57 kDa) | Santa Cruz Biotech | Sc-6260 | Mouse IgG1 | Unknown | 1:2K | |
| 2nd antibodies | ||||||
| Anti-rabbit IgG-HRP | Southern Biotech | 4050-05 | Goat IgG | 1:10-20K | ||
| Anti-mouse IgG1-HRP | Southern Biotech | 1070-05 | Goat IgG | 1:10K | ||
| Anti-goat IgG-HRP | Southern Biotech | 6160-05 | Rabbit IgG | 1:10K | ||
| Recombinant TSPO | ||||||
| Human TSPO, N-His | Creative BioMart | TSPO-3462H | / | / | 0.1 ng | 21 kDa |
| Human TSPO, N-His/GST | Creative BioMart | TSPO-1696H | / | / | <1 ng | 48.8 kDa (crude lysate) |
| Human TSPO, C-Myc/DDK | Origene | TP320107 | / | / | 40 ng | 23 kDa |
| Mouse TSPO, N-His | Jean J. Lacapere | Gift | / | / | 1 ng | 21 kDa |
| Mouse TSPO, C-Myc/DDK | Origene | TP605624 | / | / | NA | 23 kDa |
| Rat TSPO, C-Myc/DDK | Origene | TP605625 | / | / | NA | 23 kDa |