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. 2020 May;87:102188. doi: 10.1016/j.ceca.2020.102188

Fig. 4.

Fig. 4

Partial de-esterification of Mag-Fluo-4 AM provides heterogenous indicator with low Ca2+ affinity. (A) Ca2+-dependent change in the fluorescence of Mag-Fluo-4 AM (0.1 μM) or Mag-Fluo-4 (0.1 μM) after incubation (15 min at 37 °C) with the indicated concentrations of carboxylesterase. (B) Effects of carboxylesterase concentration on the fluorescence intensity measured at saturating [Ca2+]m (5 M). (C, D) Effects of carboxylesterase concentration on pKDCa (C) and h (D). (E) Ca2+-dependent change in the fluorescence of Mag-Fluo-4 AM (0.1 μM) after incubation (37 °C) with carboxylesterase (20 U/mL) for the indicated times. (F) Effects of incubation duration on the fluorescence intensity measured at saturating [Ca2+]m (5 M). (G, H) Effects of incubation duration on pKDCa (G) and h (H). All results show means ± SEM, n = 3 independent experiments each with duplicate determinations; individual values are also shown in B-D and F-H.