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. 2020 May;87:102188. doi: 10.1016/j.ceca.2020.102188

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — Partial de-esterification of Fluo-4 AM provides heterogenous indicator with low Ca²⁺ affinity. (A) Ca²⁺-dependent change in the fluorescence of Fluo-4 AM (0.1 μM) or Fluo-4 (0.1 μM) after incubation (15 min at 37 °C) with the indicated concentrations of carboxylesterase. Results show mean ± SD from 1 experiment with duplicate determinations. (B-D) Effects of carboxylesterase concentration on the fluorescence intensity measured at saturating [Ca²⁺]_m (100 mM), pK_D^Ca (C) and h (D). Results are means from 1 experiment with duplicate determinations.