Figure 4.
SUMOylated BZR1 Promotes BR-Mediated Plant Growth
(A) Representative image of hypocotyl lengths of 6-day-old seedlings of Col-0, bzr1-1D, transgenic proBZR1::BZR1-GFP, and transgenic proBZR1::BZR12K/R-GFP grown on ½ Murashige and Skoog.
(B and C) Representative image of hypocotyl lengths of 6-day-old seedlings of Col-0, bzr1-1D, transgenic proBZR1::BZR1-GFP, and transgenic proBZR1::BZR12K/R-GFP grown on BRZ (2 μM) (B) and BL (1 μM) (C) medium, respectively.
(D and E) Quantification of the ratio of hypocotyl lengths of BRZ treated (D) and BL treated (E) with reference to untreated seedlings in the dark.
(F) Representative image of root lengths of 12-day-old young adult plants of Col-0, bzr1-1D, transgenic proBZR1::BZR1-GFP, and transgenic proBZR1::BZR12K/R-GFP grown on ½ Murashige and Skoog.
(G and H) Representative image of root lengths of 12-day-old young adult plants of Col-0, bzr1-1D, transgenic proBZR1::BZR1-GFP, and transgenic proBZR1::BZR12K/R-GFP grown on BRZ (2 μM) (G) and BL (1 μM) medium (H), respectively.
(I and J) Quantification of the ratio of root growth of BRZ treated (D) and BL treated (E) with reference to untreated plants.
Scale bar, 1 cm.
Error bars indicate SE (n = 20). Asterisks indicate significant differences from Col-0. See also Figure S4.