REAGENT or RESOURCES	SOURCE	IDENTIFIER
Antibodies
GFP-Trap	Chromotek	Cat# GFP-Trap-MA
Rabbit Polyclonal anti-SUMO1 antibody	[27]	N/A
Rabbit Monoclonal anti-GFP antibody	Abcam	Cat#Ab6556; RRID: AB_305564
Monoclonal anti-HA antibody	Roche	Cat#118674223001; RRID: AB_390918
Mouse Monoclonal anti-His antibody	Sigma Aldrich	Cat#H1029; RRID: AB_2687993
Rat Monoclonal anti-GST antibody	Sigma Aldrich	Cat#SAB4200055; RRID: AB_10603625
Goat anti-rabbit IgG-HRP secondary antibody	Sigma-Aldrich	Cat#A0545; RRID: AB_2279879
Goat anti-mouse IgG-HRP secondary antibody	Sigma-Aldrich	Cat#A9917; RRID: AB_954556
Rabbit anti-rat IgG-HRP secondary antibody	Sigma-Aldrich	Cat#A5795; RRID: AB_956022
Bacterial Strains
Escherichia coli DH5α	NEB	Cat #C2987K
Escherichia coli BL 21	NEB	Cat #C2530H
Agrobacterium tumefaciens (strain GV3101)	N/A	N/A
Chemicals
Murashige and Skoog Basal Medium	Duchefa	Cat#M0233
Agar	Melford	Cat#A20250
LB-Agar	Melford	Cat#L24033
BASTA	Sigma-Aldrich	Cat#45520
IPTG	Sigma-Aldrich	Cat#I6758
Glutathione Sepharose 4B	GE Healthcare	17-0969-01
Cycloheximide	Sigma-Aldrich	Cat#C4859
RNeasy Kit	QIAGEN	Cat#74904
Ponceau S	Sigma-Aldrich	Cat#P7170-1L
Acrylamide/Bis-acrylamide, 30% solution	Sigma-Aldrich	Cat#A3699
Ammonium persulfate	Sigma-Aldrich	Cat#A3678
N,N,N,N-Tetramethylethylenediamine	Sigma-Aldrich	Cat#T9281
Magnesium chloride hexahydrate	Merck	Cat#105833
Glycerol	Merck	Cat#104098
cOmplete, EDTA-free Protease Inhibitor Cocktail	Roche	Cat#4693132001
2-mercaptoethanol	Sigma-Aldrich	Cat#M3148
DTT	Malford	Cat#D11000
Critical Commercial Assay
RNeasy Plant Mini Kit	QIAGEN	Cat#74904
SYBR Green	Sigma-Aldrich
SuperScript III Reverse Transcriptase	Thermo Fisher	Cat#18080093
Zymoclean Gel DNA Recovery	Zymo research	Cat#D4007
ZymoPURE II Plasmid Kits	Zymo research	Cat#D4201
Experimental Models
Arabidopsis ots1 ots2	[27]	N/A
Arabidopsis ulp1a	This Study	N/A
Arabidopsis bzr1-1D	This Study	N/A
Arabidopsis 35S::BZR1-GFP	This Study	N/A
Arabidopsis 35S::BZR1K280/320R-GFP	This Study	N/A
Arabidopsis proBZR1::BZR1-GFP	This Study	N/A
Arabidopsis proBZR1::BZR1K280/320R-GFP	This Study	N/A
Arabidopsis 35S::ULP1-HA	This Study	N/A
Oligonucleotides
Primers are listed in Table S1	This Study	N/A
Constructs
Constructs used are listed in Table S2
Software and Algorithms
ImageJ	NIH, USA	http://rsb.info.nih.gov/ij
GraphPad Prism6	Graph Pad Software	https://www.graphpad.com