Figure 1.
Liquid-like G3BP1 SGs Form by Heterotypic Phase Separation in Cells
(A) Fluorescence images from a LLSM time-lapse movie of an oxidatively stressed HeLa cell expressing G3BP1-mCherry. Inset: G3BP1 foci. Scale bar, 10 μm.
(B) Analysis of the mean averages of G3BP1 foci within an individual cell as a function of time.
(C) Left: normalized number of G3BP1-positive SGs in individual cells as a function of time (every 100th data point is shown). Center: correlation of SG assembly onset as a function of G3BP1 mean fluorescence intensity. Right: correlation of G3BP1-positive SG number as a function of G3BP1 mean fluorescence intensity. Dashed lines are linear regression as a guide.