Figure 5.

Interactions between RG-Rich and Autoinhibitory Acidic IDRs Regulate G3BP1 Phase Separation

(A) Conformational snapshot of G3BP1 from an excluded volume (EV) simulation (instantaneous R_H, ∼11 nm).

(B) Normalized distance between any pair of residues in G3BP1(ΔNTF2) simulations. Cooler colors are closer together, and warmer colors are farther apart. Dashed lines delineate domains.

(C) R_H for G3BP1 dimers, inferred from FCS measurements and EV simulations of full-length dimeric G3BP1 with generic, self-avoiding descriptions for the IDRs.

(D) R_H of G3BP1(WT) as a function of KCl concentration, determined by DLS. Fit shown with a dashed line.

(E) R_H of G3BP1 variants, determined by FCS.

(F) R_H of G3BP1(WT) at pH 6 and pH 7.5, determined by DLS.

(G) Oligomeric species of G3BP1(WT) at pH 6 or pH 7.5, detected by DLS. Shown is the mean average (n = 30).

(H) Analytical gel filtration of G3BP1(WT) and G3BP1(ΔRG) on A(60) RNA as a function of RNA concentration.

Significance levels: ^∗ < 0.05, ^∗∗ < 0.01, ^∗∗∗ < 0.001. See also Figure S5.