Figure S7.
A: Schematic representation of G3BP1 and G3BP2 domains, with the content of Arginine residues within the RG-rich region highlighted. Below, a sequence alignment of G3BP1 and G3BP2 RG-rich region. Arginine residues depicted in cyan. B: The sequence conservation of 339 G3BP1/2 orthologs was determined. The two folded domains are highly conserved, while the IDRs show virtually no absolute sequence conservation, except for a highly conserved binding motif in the PxxP domain which we speculate mediates recruitment of other key component(s). C: Despite having almost no sequence conservation, the region that would correspond to the acidic IDR (magenta) is almost uniformly highly acidic across all orthologous G3BP1/2 sequences.