REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-G3BP1	Thermo Fisher Scientific	Cat#PA5-29455; RRID:AB_2546931
Goat anti-eIF3η	Santa Cruz Biotechnology	Cat#N-20; RRID:AB_671941
Donkey anti-goat 647	Invitrogen	Cat#A21447; RRID:AB_141844
Donkey anti-rabbit 488	Invitrogen	Cat#A21206; RRID:AB_2535792
Rabbit anti-phospho-G3BP1 (pS149)	Sigma Aldrich	Cat#G8046; RRID:AB_1840867
Mouse anti-puromycin (clone 12D10)	Merck Millipore	Cat#MABE343; RRID:AB_2566826
Rabbit anti-eIF2a(Ser52)	Thermo Fisher Scientific	Cat#44-728G; RRID:AB_2533736
Mouse anti-eIF2a	Santa Cruz Biotechnology	Cat#sc-133132; RRID:AB_1562699
Goat anti-EGFP	In house	N/A
Bacterial and Virus Strains
BL21-AI One Shot Chemically Competent E. coli	Thermo Fisher Scientific	Cat#607003
Chemicals, Peptides and Recombinant Proteins
Ubc9WT-6xHis	Mateju et al., 2017	O-3299
Ubc9ts-6xHis	Mateju et al., 2017	O-3298
GFP-G3BP1(WT)	This paper	L-515
GFP-G3BP1(ΔNTF2)	This paper	TH1321
GFP-G3BP1(ΔRG)	This paper	TH0999
GFP-G3BP1(ΔE1)	This paper	L-663
GFP-G3BP1(ΔE2)	This paper	L-664
GFP-G3BP1(ΔE1ΔE2)	This paper	L-667
GFP-G3BP1(S149A)	This paper	L-548
GFP-G3BP1(S149E)	This paper	L-549
GFP-G3BP1(S149A/S232A)	This paper	L-686
SNAP-G3BP1(WT)	This paper	L-587
mCherry-Caprin1	This paper	TH1232
GFP-G3BP2(WT)	This paper	TH1434
GFP	This paper	D292
GFP-hnRNPA1L	Wang et al., 2018	TH1261
GFP-hnRNPA2B1	Wang et al., 2018	TH1252
FUS-GFP	Wang et al., 2018	TH1204
TDP43-GFP	Wang et al., 2018	TH1083
GFP-hnRNPA1L2	Wang et al., 2018	TH1275
GFP-hnRNPA0	Wang et al., 2018	TH1250
GFP-hnRNPAB	Wang et al., 2018	TH1251
GFP-hnRNPA1S	Wang et al., 2018	TH1164
GFP-hnRNPDL	Wang et al., 2018	TH1254
GFP-hnRNPA3	Wang et al., 2018	TH1202
GFP-TIAL1	Wang et al., 2018	TH1257
GFP-TIA1	Wang et al., 2018	TH1256
GFP-PSPC	This paper	TH1255
SFPQ-GFP	This paper	TH1263
RNaseOUT	Invitrogen	Cat#10777019
Nucleotides: ATP, CTP, GTP, and UTP	Roche	Cat#11277057001
DNase I	New England Biolabs	Cat#M0303S
E. coli PolyA Polymerase	Thermo Fisher Scientific	Cat#AM1350
HCS CellMask Blue Stain	Invitrogen	Cat#H32720
Hoechst 33342, Trihydrochloride, Trihydrate – 10 mg/ml solution in water	Invitrogen	Cat#H3570
Blasticidin S HCl	Invitrogen	Cat#R21001
Lipofectamine 2000	Life Technology	Cat#11668-019
Opti-MEM	GIBCO	Cat#31985062
DMEM, high glucose, GlutaMAX Supplement, pyruvate	GIBCO	Cat#31966021
DMEM, high glucose, no glutamine, no phenol red	GIBCO	Cat#31053028
cOmplete Protease Inhibitor Cocktail	Roche	Cat#11836145001
cOmplete, EDTA-free Protease Inhibitor Cocktail	Roche	Cat#05056489001
RNase A	PanReac AppliChem	Cat#A2760
Polyethylene glycol 20000	Merck	Cat#817018
SNAP-Surface Alexa Fluor 546	New England Biolabs	Cat#S9132S
T4 RNA ligase 1	New England Biolabs	Cat#M0204S
pCp-Cy5	Jena Bioscience	Cat#NU-1706-CY5
Lambda Protein Phosphatase	New England Biolabs	Cat#P0753S
Nonidet P 40	Sigma-Aldrich	Cat#74385
2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane; 90% 6-9 PE-units	Abcr GmbH	Cat#AB111226
Sodium hydrogen arsenate heptahydrate, ASC, 98.0-102.0%	Alfa Aesar	Cat#33373
Critical Commercial Assays
RNeasy Mini Kit (50)	QIAGEN	Cat#74104
mMESSAGE mMACHINE T7 Transcription Kit	ThermoFisher	Cat#AM1344
Poly(A) Tailing Kit	ThermoFisher	Cat#AM1350
Microspin G-25 Columns	GE Healthcare	Cat#GE27-5325-01
Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO	ThermoFisher	Cat#89849
Experimental Models: Cell Lines
G3BP1(WT) BAC in HeLa Kyoto cell line	Mateju et al., 2017	MCB_ky_7510 (C-terminal mCherry tag; FACS sorted)
U2OS	Kedersha et al., 2016; Laboratory of Paul Anderson	N/A
U2OS G3BP1/2 knock out cell line	Kedersha et al., 2016; Laboratory of Paul Anderson	N/A
Sf9 cells	Expression Systems	Cat#94-001F
Oligonucleotides
polyA(60) RNA	This paper; Eurofins Genomics	N/A
Poly(A)	Sigma-Aldrich	Cat#10108626001
Poly(C)	Sigma-Aldrich	Cat#P4903
Poly(G)	Sigma-Aldrich	Cat#P4404
Poly(U)	Sigma-Aldrich	Cat#P9528
Human spinal cord poly(A)+ RNA	Clontech	Cat#636142
NEAT1 RNA	This paper	N/A
5-loop RNA	This paper	N/A
Ribosomal RNA	Bioworld	Cat#11020001-2
Recombinant DNA
pCRII-TOPO-hNEAT1	Clemson et al., 2009; Laboratory of Archa Fox	Addgene Plasmid #61518
pSCA-5loopPlus5	This paper	L-480
Software and Algorithms
Fiji	NIH	https://fiji.sc/
NCPR	Alan Bleasby European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK	http://www.bioinformatics.nl/cgi-bin/emboss/charge
PLAAC	Lancaster et al., 2014	http://plaac.wi.mit.edu/
IUPred	Dosztányi et al., 2005	https://iupred2a.elte.hu
KNIME	KNIME.com AG	https://www.knime.com
R / RStudio	R Core Team	https://www.r-project.org/ https://www.rstudio.com/