. 2020 Apr 10;25(7):1752. doi: 10.3390/molecules25071752

Table 2.

Antimicrobial efficacy of singular treatment of essential oils in microbial suspension.

Medium	Treatment Conditions	Target Microorganisms	Singular Treatment ¹	Antibacterial Effects (Log Reduction; Log CFU/g or mL)	References
0.85% saline	37 °C, 10 min	Escherichia coli O157:H7	CAR/EUG/RA/TC/TM/VNL 1 mM	negligible (ca. <1)	[25]
0.85% saline	22 °C, 5 min	Escherichia coli Staphylococcus aureus	CAR/TM 2 mM	negligible (ca. <1)	[45]
Deionized water	22 °C, 10 min	Escherichia coli Listeria monocytogenes Staphylococcus aureus	TM 2 mM	negligible (ca. <1)	[44]
Deionized water	22 °C, 10 min	Escherichia coli Listeria monocytogenes Staphylococcus aureus	CAR 2 mM	1–2	[44]
Deionized water (with 10 μg/μL Tween 80)	1 min ²	Escherichia coli O157:H7 Listeria innocua	CAR 0.875 μg/mL	>4	[46]
0.1% peptone water	37 °C, 30 min	Escherichia coli O157:H7	cinnamon bark oil 0.0625%	>3	[66]
		Escherichia coli O157:H7	cinnamon leaf oil 0.0625%	>3
		Salmonella Typhimurium	cinnamon bark oil 0.0625%	4
		Salmonella Typhimurium	cinnamon leaf oil 0.0625%	>3
Brain heart infusion broth	4 °C, 8 h	Escherichia coli	Mentha arvensis L. oil 0.625 μL/mL	>5	[49]
	4 °C, 8 h	Salmonella Enteritidis	Mentha piperita oil 5 μL/mL	>5	[49]
	37 °C, 8 h	Escherichia coli	armoise oil 0.10%	>8.0	[50]
	37 °C, 8 h	Escherichia coli	clove oil 0.10%	>7.5	[50]
Butterfield’s phosphate buffer	2 min ²	Escherichia coli Salmonella Typhimurium Staphylococcus aureus	orange oil 10%	7	[47]
Fish peptone broth	4 °C, 12 d	Listeria monocytogenes	Bunium persicum (Black zira) oil 0.20%	3.2	[52]
Luria-Bertani broth	22 °C, 3 h	Escherichia coli O157:H7 Cronobacter sakazakii	TM 150 μg/mL	1	[59]
			CAR 300 μg/mL	1
			TC 350 μg/mL	1
Mueller-Hinton broth	4 °C, 24 h	Campylobacter jejuni	rosemary extract 310 μg/mL	7	[51]
Mueller-Hinton broth	37 °C, 0.17 h	Escherichia coli Staphylococcus aureus	oregano oil 0.596 μg/mL	5	[67]
Phosphate-buffered saline	37 °C, 72 h	Salmonella Typhimurium	bark cinnamon oil 0.5%	>9	[48]
	37 °C, 8 h	Listeria monocytogenes	leaf cinnamon oil 0.5%	>9	[48]
	2 min ²	Vibrio parahaemolyticus	orange oil 10%	7	[47]
Tryptic soy broth	32 °C, 24 h	Listeria monocytogenes	bark cinnamon oil 313 ppm	2.0	[49]
	32 °C, 24 h	Listeria monocytogenes	TM 625 ppm	5.3	[49]
	37 °C, 16 h	Escherichia coli O157:H7	noni oil 4 μL/mL	>8	[54]
	37 °C, 16 h	Salmonella enterica	noni oil 4 μL/mL	>8	[54]
	32 °C, 24 h	Escherichia coli O157:H7	clove oil 600 μg/mL	>5	[55]
		Salmonella Typhimurium	garlic/cinnamon oil 600 μg/mL	3
		Listeria monocytogenes	garlic/clove oil 400 μg/mL	>5
	37 °C, 24 h	Escherichia coli	Eucalyptus globulus oil 5 μL/mL	8.7	[56]
		Salmonella Enteritidis	Eucalyptus globulus oil 7.5 μL/mL	8.1
		Bacillus cereus	Eucalyptus globulus oil 5 μL/mL	9.0
		Staphylococcus aureus	Eucalyptus globulus oil 5 μL/mL	9.0
	35 °C, 3 h	Escherichia coli Staphylococcus aureus	TM 300 ppm	4–5	[57]
		Listeria monocytogenes	TM 500 ppm	4–5
		Salmonella Typhimurium	TM 500 ppm	4–5
	37 °C, 24 h	Escherichia coli	EUG/VNL 125 μg/mL	7	[58]

¹ Abbreviation of essential oils: carvacrol (CAR), eugenol (EUG), β-resorcylic acid (RA), trans-cinnamaldehyde (TC), thymol (TM), and vanillin (VNL). ² Treatment temperature was not indicated in the previous reports.