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. 2020 Apr 1;6(4):535–545. doi: 10.1021/acscentsci.9b01222

Figure 2.

Figure 2

Liposome protein corona fingerprints resolved by gel electrophoresis. (a) Schematic representation of the centrifugation protocol. (b) Schematic representation of the photoaffinity protocol. (c) SDS-PAGE of human serum, stained with Coomassie Blue. (d) SDS-PAGE of the liposome protein coronas, as well as captured proteins of a liposome free control sample, isolated by centrifugation and stained with Coomassie Blue. (e) SDS-PAGE of the liposome protein coronas, isolated by photoaffinity method, visualizing Cy5 labeled lipid–protein conjugates by in-gel fluorescence. Unique bands highlighted with red arrows. (below) Coomassie Blue loading controls displayed as cropped images. Complete gels displaying all controls and complete Coomassie Blue stained gels displayed in Figure S3.