a,b, Normalized nucleotide frequencies relative to mapped
positions of sequences from Ribose-seq library of
rnh201Δ (KK-100) cells. Position 0 is the rNMP.
c,d, Zoom-out of frequencies. e, Ratios of rNMPs
on newly synthesized leading to lagging strand for all ribose-seq libraries.
Early-firing ARSs selected by their replication timing
(Trep) were investigated for two different
flanking sizes. f,g, Normalized nucleotide frequencies relative to
mapped sequence positions in leading (f) and lagging
(g) strands from the rnh201Δ (KK-100)
library. ARSs with a Trep of no longer than 25 min
were selected with flanking size of 10 kb. h–k, Normalized
nucleotide frequencies relative to mapped sequence positions from a
pol2-4 rnh201Δ (KK-107) library.
Reads were mapped to (a,c,h) nuclear genome, (b,d,i)
mitochondrial genome, (f,j) leading strand or (g,k)
lagging strand.