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. Author manuscript; available in PMC: 2020 Apr 24.
Published in final edited form as: Dev Dyn. 2019 Nov 20;249(2):245–261. doi: 10.1002/dvdy.130

Figure 4.

Figure 4.

A comparison of etv2ci32Gt+/−; UAS:GFP, TgBAC(etv2:GFP), Tg(−2.3etv2:GFP) fluorescence pattern (in kdrl:mCherry background, top row) and etv2 mRNA expression analyzed by in situ hybridization (ISH) at 24 hpf. etv2ci32Gt+/−; UAS:GFP expression is apparent throughout the entire vasculature, red blood cells (RBC) and macrophages (MF). TgBAC(etv2:GFP) shows similar expression pattern, and also shows non-specific expression in the neural tube. (A-C) Merged mCherry and GFP channels; (D-F) GFP channel; (G,H) magnified images of the trunk region in (A,B). (I) ISH analysis for etv2 mRNA expression at 24 hpf. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessels.