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. Author manuscript; available in PMC: 2020 Apr 24.
Published in final edited form as: Dev Dyn. 2019 Nov 20;249(2):245–261. doi: 10.1002/dvdy.130

Figure 5.

Figure 5.

A comparison of etv2ci32Gt+/−; UAS:GFP, TgBAC(etv2:GFP), Tg(−2.3etv2:GFP) fluorescence pattern (in kdrl:mCherry background) at 48 hpf (A-I) and 72 hpf (J-R). etv2ci32Gt+/−; UAS:GFP expression is apparent throughout the entire vasculature and in lymphatic progenitors (parachordal lymphangioblasts, PLs). TgBAC(etv2:GFP) shows similar expression pattern, and also shows non-specific expression in the neural tube. Vascular endothelial expression in Tg(−2.3etv2:GFP) line is downregulated after 48 hpf and is very weak at 72 hpf, while non-specific epithelial expression is apparent. (A-C, J-L) Merged mCherry and GFP channels; (D-F, M-O) GFP channel; (G-I, P-R) magnified images of the trunk region in (A-C, J-L). DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessels, SIV, subintestinal vessel; DLAV, dorsal longitudinal anastomotic vessel; CCV, common cardinal vein.