Effect of photothermolysis mediated by CuS NPs plus PW laser in combination with αPD-1 on tumor infiltration of T cells, antigen-presenting cells, and myeloid cells in B16-OVA tumor. (A) Representative flow cytometry images. (B, C) Analysis of tumor infiltration of (B) T cells and (C) macrophages (CD45+CD11b+CD169+), DCs (CD45+CD11b+CD11c+), and MDSCs (CD45+CD11b+Gr1+). Data are presented as the mean ± standard error of the mean (n = 3). *p<0.05, **p<0.01, ***p<0.001.