Survival of mice re-inoculated with ID8-ip1-Luc ovarian cancer cells after combined photothermolysis and immunotherapy, with one or two rounds of photothermolysis. (A) Experimental design diagram. (B) Kaplan-Meyer survival curves for mice monitored for up to 182 days after institution of laser treatment. ****p<0.0001 for combination treatment (20 OD CuS NPs + PW laser 2 min + PG-CpG + αPD-1 groups) compared to photothermolysis alone and untreated control group (log-rank Mantel-Cox test; n = 9 for the control, one round of photothermolysis, and one round of photothermolysis + PG-CpG + αPD-1 groups; n = 8 for the two rounds of photothermolysis and two rounds of photothermolysis + PG-CpG + αPD-1 groups), i.p., intraperitoneal; i.v., intravenous; s.c., subcutaneous.