KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Dynabeads M-280 Sheep Anti-Rabbit IgG | Invitrogen | #11203D; RRID: AB_2783009 |
| Dynabeads M-280 Sheep Anti-Mouse IgG | Invitrogen | #11202D; RRID: AB_2783640 |
| Anti-HA | Millipore | #05-904; RRID: AB_11213751 |
| Anti-cMyc | EMD Millipore | #05-724; RRID: AB_11211891 |
| Anti-GFP | Abcam | #ab290; RRID: AB_303395 |
| Experimental Models: Organisms/Strains | ||
| Library of random sheared yeast genomic DNA in pACTII | This paper | N/A |
| Y, a, mps3Δ240-430 | This paper | MCY 1456 |
| X, alpha, csm4::LEU2 | This paper | MCY 1536 |
| Y, a, csm4::LEU2 | This paper | MCY 1539 |
| Y, a, mps2ΔC6-TRP1 | This paper | MCY 1885 |
| X, alpha, mps2ΔC6-TRP1 | This paper | MCY1886 |
| X, alpha, MYO5-GFP-TRP1 | This paper | MCY 2037 |
| Y, a, MYO5-GFP-TRP1 | This paper | MCY 2038 |
| X, alpha, myo3::TRP1 myo5::TRP1 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 | This paper | MCY 2083 |
| Y, a, myo3::TRP1 myo5::TRP1 leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1- GFP/lacI at LYS2 | This paper | MCY 2092 |
| X, a, MYO1-GFP-TRP1 | This paper | MCY 2134 |
| Y, alpha, MYO1-GFP-TRP1 | This paper | MCY 2135 |
| Y, a, kanMX-CLB2-Ubi-MYO1 | This paper | MCY 2146 |
| X, alpha, kanMX-CLB2-Ubi-MYO1 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MCY 2147 |
| X, a, GFP-myc-MPS2-URA3 csm4::LEU2 | This paper | MCY 2213 |
| Y, alpha, GFP-myc-MPS2-URA3 csm4::LEU2 | This paper | MCY 2214 |
| X, a, GFP-myc-MPS2-URA3 ndj1::TRP1 | This paper | MCY 2215 |
| Y, alpha, GFP-myc-MPS2-URA3 ndj1::TRP1 | This paper | MCY 2216 |
| X, alpha, GFP-myc-MPS2-URA3 csm4::LEU2 ndj1::LEU2 | This paper | MCY 2217 |
| Y, a, GFP-myc-MPS2-URA3 csm4::LEU2 ndj1::LEU2 | This paper | MCY 2218 |
| X, alpha, GFP-myc-MPS2-URA3 | This paper | MCY 2219 |
| Y, a, GFP-myc-MPS2-URA3 | This paper | MCY 2220 |
| Y, a, myo4::TRP1 leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MCY 2241 |
| X, alpha, myo4::TRP1 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MCY 2244 |
| X, alpha, GFP-myc-mps2ΔC6-TRP1 | This paper | MCY 2249 |
| Y, a, GFP-myc-mps2ΔC6-TRP1 | This paper | MCY 2251 |
| Y a kanmx-GAL-HA-MYO3 | This paper | MCY 2387 |
| X, alpha, kanmx-GAL-HA-MYO3 | This paper | MCY 2388 |
| X, alpha, MYO4-GFP-TRP1 | This paper | MCY 2443 |
| Y, a, MYO4-GFP-TRP1 | This paper | MCY 2444 |
| Y, alpha, mps3Δ240-430 mps2ΔC6-TRP1 | This paper | MCY 2484 |
| X, a, mps3Δ240-430 mps2ΔC6-TRP1 | This paper | MCY 2486 |
| X, alpha, GAL-MYO2-DN-LEU2 GAL4-ER-URA3 | This paper | MCY 2541 |
| Y, a, GAL-MYO2-DN-LEU2 GAL4-ER-URA3 | This paper | MCY 2543 |
| Y, a, mps2ΔC6-TRP1 leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MCY 2722 |
| X, alpha, mps2ΔC6-TRP1 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MCY 2728 |
| Y, alpha, GAL4-ER-URA3 GAL-GST-MYO2-TRP1 leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MCY 3190 |
| X, a, GAL4-ER-URA3 GAL-GST-MYO2-TRP1 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MCY 3192 |
| Y, alpha, GAL4-ER-URA3 GAL-MYO2-DN-TRP1 ndt80::HPH leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MCY 3245 |
| X, a, GAL4-ER-URA3 GAL-MYO2-DN-TRP1 ndt80::HPH Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MCY 3247 |
| X, a, ade5°canR CYHs his7-2 leu2 lys2-1° met13-d trp1–63 tyr1-1(−2?) ura3-13(PER1+) GAL+ (wild-type) | This paper | MDY 1480 |
| Y, alpha, ade5°canR CYHs his7-2 leu2 lys2-1° met13-d trp1–63 tyr1-1(−2?) ura3-13 (PER1+) GAL+ (wild-type) | This paper | MDY 1508 |
| Y, alpha, leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MDY 1567 |
| X, a, Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MDY 2426 |
| X, a, csm4::LEU2 Pdmc1-I/GFP-URA3 at Ura3, Pcyc1-I/GFP-URA3 atCyc1, Laco256-LEU2 at Chr4telo | This paper | MDY 2609 |
| X, alpha, mps3Δ240-430 | This paper | MDY 2661 |
| Y, alpha, csm4::LEU2 leu1 TUB1/GFP lacO256-LEU2-IVRtelo P-DMC1-GFP/lacI at LYS2 | This paper | MDY 2778 |
| X, a, Myo2-GFP-TRP1 mps2ΔC6-TRP1 | This paper | MDY 3554 |
| Y, alpha, Myo2-GFP-TRP1 mps2ΔC6-TRP1 | This paper | MDY 3557 |
| X, alpha, GFP-myc-MPS2-URA3 Mps3-HA-TRP1 | This paper | MDY 4027 |
| Y, a, GFP-myc-MPS2-URA3 Mps3-HA-TRP1 | This paper | MDY 4030 |
| Software and Algorithms | ||
| ImageJ | [66] | https://imagej.nih.gov/ij/ |
| AxioVision | Zeiss | https://www.zeiss.com/ |
| Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| OMRFQUANT | Our laboratory | N/A |