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. 2020 Jan 2;13(3):460–470. doi: 10.1038/s41385-019-0246-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Analysis of IL-17A or IFNγ expressing CD4⁺ T cell responses in SAg-Sau stimulated tonsillar MNCs treated with IL-10 neutralizing antibody or isotype control. MC is unstimulated media control. Zebra plots were gated on CD4⁺ T cells and numbers in top right quadrants indicate the percentages of Th1 (a) or Th17 (b) within CD4⁺ T cell population. Data was analyzed using paired t-test with p values indicated, n = 7. c, d CD69⁺ cell depleted tonsillar MNCs were stimulated with SAg-Sau CCS in the presence or absence of recombinant IL-10 (10 ng/ml) for 48 h. Ctrl is the stimulation control. c Numbers in top right quadrants of the zebra plots indicate the percentage of Th17 in CD4⁺ T cells. Results of 8 individual samples were analyzed and summarized in symbol and line plot. d The percentage of IL-17A⁺IFNγ^-, IL-17A⁺IFNγ⁺, and IL-17A^-IFNγ⁺ cells within CD4⁺ T cell population is shown in top left, top right and bottom right quadrants of the zebra plots. Summarized data is displayed in symbol and line plot, n = 5.