Table 1.
qPCR Primers (synthesized by Sigma Aldrich).
| Gene | Primers | Tm (°C) | Product size (bp) | |
|---|---|---|---|---|
| Human il12a NM_000882 | Forward | 5’-TGCCTTCACCACTCCCAAAACCTGCTGA-3’ | 70.0 | 154 |
| Reverse | 5’-ATGGTAAACAGGCCTCCACTGTGCTGGT-3’ | 69.6 | ||
| Human ebi3 NM_005755.2 | Forward | 5’-AAACTCCACCAGCCCCGTGTCCTTCATT-3’ | 69.9 | 161 |
| Reverse | 5’-CGGTGACATTGAGCACGTAGGGAGCCAT-3’ | 70.1 | ||
| Human βactin NM_001101.5 | Forward | 5’-GCTCACCATGGATGATGATATCGCCGC-3’ | 67.7 | 199 |
| Reverse | 5’-GATGCCTCTCTTGCTCTGGGCCTCGTC-3’ | 70.2 | ||
| Mouse il17a NM_010552.3 | Forward | 5’-CCAGCTGATCAGGACGCGCAAACATGAG-3’ | 69.7 | 115 |
| Reverse | 5’-TGAGGGATGATCGCTGCTGCCTTCACTG-3’ | 70.1 | ||
| Mouse il12a NM_001159424.2 | Forward | 5’-TTCCAGGCCATCAACGCAGCACTTCAGA-3’ | 70.2 | 196 |
| Reverse | 5’-TGAAGGCGTGAAGCAGGATGCAGAGCTT-3’ | 70.3 | ||
| Mouse ebi3 NM_015766.2 | Forward | 5’-GCCTCCTAGCCTTTGTGGCTGAGCGAAT-3’ | 70.3 | 133 |
| Reverse | 5’-GAGAAGATGTCCGGGAAGGGCCAGGAAG-3’ | 69.8 | ||
| Mouse βactin NM_007393.5 | Forward | 5’-TTCTTTGCAGCTCCTTCGTTGCCGGTC-3’ | 69.5 | 199 |
| Reverse | 5’-CCTTCTGACCCATTCCCACCATCACACC-3’ | 68.6 | ||