KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-MAP7 | Thermofisher | PA5-31782 |
| Mouse monoclonal anti-Tau-1 | Millipore | MAB3420 |
| Anti-chicken tau | Genetex | GTX49353 |
| Anti-mouse MAP2 | Sigma | M3696 |
| Anti-rabbit MAP9 | Invitrogen | PA5-58145 |
| Anti-mouse DCLK | Invitrogen | MA5-26800 |
| Anti-mouse DCX | Invitrogen | MA5-17066 |
| Mouse anti-alpha tubulin | Sigma Clone DM1A | T9026 |
| Rabbit anti-beta tubulin | Abcam | Ab6046 |
| Rabbit anti-MAP7 | Genetex | GTX120907 |
| Anti-rabbit MAP2 | Millipore | #AB5622 |
| Anti-mouse MAP7 | Sigma | SAB1409973 |
| Cy3 donkey anti-rabbit | Invitrogen | #A27034 |
| Cy5 donkey anti-mouse | Invitrogen | #A28180 |
| Cy3 goat anti-chicken | Invitrogen | A11039 |
| Vectashield no DAPI | Vector Laboratories | H-1000 |
| Bacterial and Virus Strains | ||
| Escherichia coli (BL21DE3) | Agilent | 200131 |
| Escherichia Coli (XL10Gold) | Agilent | 200314 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Biotinylated poly(L-lysine)-[g]-poly(ethylene-glycol) (PLL-PEG-Biotin | SuSoS AG | PLL(20)-G[3.5]-PEG(2)/PEG(3.4)-biotin(50%) |
| Streptavidin | ThermoFischer | 21135 |
| Trolox (6-hydroxy-2,5,6,7,8-tetramethylchroman-2-carbonsaure, 97%) | Acros | AC218940050 |
| 3,4-Dihydroxybenzoic Acid (Protocatechuic acid) | Sigma-Aldrich | 37580 |
| Protocatachuate 3,4-Dioxygenase from Pseudomonas sp. | Sigma-Aldrich | P8279 |
| κ-caesin from bovine milk | Sigma-Aldrich | C0406 |
| Pierce Bovine Serum Albumin, Biotinylated | Thermo-Fischer | 209130 |
| Paclitaxel | Sigma-Aldrich | T7402 |
| Pluronic F-157 | Sigma-Aldrich | P2443 |
| Glass cover slides (18x18-1.5) | Fischer | 12-541A |
| Superfrost Microscope slides | Fisher | 12-550-143 |
| Adenosine 5’-triphosphate disodium salt hydrate | Sigma-Aldrich | A2383 |
| Guanosine 5’-triphosphate sodium salt hydrate | Sigma-Aldrich | G8877 |
| Bovine Serum Albumin | Sigma-Aldrich | A2058 |
| Casein | Sigma-Aldrich | C7078 |
| Nonidet P 40 substitute (NP-40) | Sigma-Aldrich | 74385 |
| PDMS (polydimethylsiloxane, sylgard 184) | Sigma-Aldrich | 761036 |
| DNAseI | NEB | M0303L |
| Streptactin Superflow resin | Qiagen | 30002 |
| Streptactin XT Superflow resin | IBA | 2-4010-025 |
| d-Desthiobiotin | Sigma | D1411 |
| D-biotin | CHEM-IMPEX | #00033 |
| Anti-FLAG M2 Affinity gel beads | Thermofisher | A2220 |
| FuGENE 6 transfection reagent | Promega | E2691 |
| Experimental Models: Cell Lines | ||
| Primary mouse hippocampus neurons | S. Simo | |
| Flp-In T-Rex 293 Cell Line | Thermofisher | #R78007 |
| Recombinant DNA | ||
| Human Tau-2N4R | Addgene | #16316 |
| Human MAP7 | GE Dharmacon MGC Collection | #BC025777 |
| Human MAP2 | Transomics | #BC172263 |
| Human MAP9 | Transomics | #BC146864 |
| Human DCX | Addgene | #83928 |
| Mouse DCLK | Transomics | #BC133685 |
| Human KIF5B (aa 1-560) | R. Vale | |
| Human KIF1A (aa 1-393) | Addgene | #61665 |
| pEGFP-N1-HsKIF1A full-length | S. Niwa | |
| Software and Algorithms | ||
| FIJI | Schindelin et al., 2012 | https://Fiji.sc/ |
| GraphPad Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| μManager | Edelstein et al., 2010 | https://micro-manager.org/ |