Figure 4.

The AvrA C186A mutant plasmid abolishes the regulation of exogenous Beclin-1. The HCT116 cells were transfected with the indicated plasmids (200 ng/μl, 24 h incubation, n = 4). At the indicated times, the changes in the target proteins were measured. (A) The Western blot shows the expression of Beclin-1 in the HCT116 cells after transfection with the indicated plasmids. The relative density of Beclin-1 (B) was determined using Quantity One 4.6.2 software (Bio-Rad, Hercules, CA, USA). n=4, *P < 0.05 by student's t-test. (C) The Western blot shows the change in the JNK pathway markers and Beclin-1 in the HCT116 cells after transfection with the AvrA wild-type and AvrA mutant plasmids. These results shown are representative of four independent experiments.