Figure 5.
Salmonella Enteritidis AvrA changes the levels of Beclin-1 in mouse intestinal organoids. Salmonella Enteritidis infection and invasion in the mouse organoids. (A) The Western blot shows the expression of Beclin-1, P62, LC3, and the JNK pathway markers in the organoids after infection with wild-type Salmonella Enteritidis or AvrA mutant or AvrA-complemented strains (MOI:1:1, 1.5 h incubation before harvested, n = 3). The relative density of Beclin-1 (B), P62 (C), and LC3 II/LC3 I (D) was determined using Quantity One 4.6.2 software (Bio-Rad, Hercules, CA, USA). n = 3, *adjusted P < 0.05, **adjusted P < 0.01, #unadjusted P < 0.05 by ANOVA test. (E) The representative images of the immunostaining of P62 in the organoids after infection with wild-type Salmonella Enteritidis or AvrA mutant or AvrA-complemented strains (MOI:1:1, 1.5 h incubation).