Figure 4.

Effect of bicyclol on hepatic p62, Nrf2, and Keap1 mRNA expression (A–C) and Nrf2 and Keap1 protein expression in 24 and 48 h CCl₄-triggered mice (D–F). Mice were intraperitoneally injected a mixture of CCl₄ (50%) and oil (50%) at a dose of 2 ml/kg body weight. Mice were given bicyclol 200 mg/kg by gavage for three times in 1 day 1 h prior to CCl₄ challenge. Results are presented as mean ± SD for five mice per group. Significantly different (*p < 0.05, ***p < 0.001, ^#p < 0.05, ^###p < 0.001) from the control group. Significantly different (^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001) from the CCl₄ 24 h group. Significantly different (^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001) from the CCl₄ 48 h group.