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. 2020 Apr 24;11:2027. doi: 10.1038/s41467-020-15656-4

Fig. 3. WLS is required for Wnt secretion from OPCs.

Fig. 3

a brain primary OPCs growing in the growth medium (GM) were transfected with Wls-shRNA and Wnt7a plasmids, and OPC mRNA and culture medium were collected for analysis 48 hours (h) after the first Wls-shRNA transfection. b RT-qPCR assay of Wls and Wnt7a mRNA in transfected primary OPCs. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001. F(3,8) = 17.41, P = 0.007 Wls, F(3,8) = 33.82, P < 0.0001 Wnt7a. n = 3 each group. c ELISA measurement of Wnt7a protein concentration in the GM in the absence and presence of OPCs in the dish with Wls-shRNA and Wnt7a transfection. One-way ANOVA followed by Tukey’s multiple comparisons, ***P < 0.001, ns not significant. F(4,10) = 21.02, P < 0.0001. Note that Wnt7a concentration in the GM in the presence of primary OPCs is not statistically different from that in the GM in the absence of primary OPCs. n = 3 each group. d RT-qPCR assay of Wnt target genes Axin2 and Sp5 in OPCs. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01. F(3,8) = 9.632, P = 0.0049 Axin2, F(3,8) = 6.965, P = 0.0128 Sp5. n = 3 each group. Data are shown as mean ± s.d. Source data of bd are provided as a Source Data file.