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. 2020 Apr 23;11(4):269. doi: 10.1038/s41419-020-2474-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b MTT assays (a) and EdU assays (b) showed miR-4476 inhibitor suppressed U87 and LN229 cells proliferation and silencing APC reversed these effects; scale bar = 50 μm. c, d Transwell assays (c) and Boyden assays (d) showed silencing APC restored the migration and invasion ability suppressed by miR-4476 inhibitor in U87 and LN229 cells; scale bar = 75 μm. e, f Western blot of endogenous N-cadherin, E-cadherin, snail, slug, APC, β-catenin, c-Jun, c-myc, and CCND1 protein expression levels in U87 and LN229 cells treated as indicated. β-actin and GAPDH served as loading controls. Mean ± S.D., *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.