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. 2020 Apr 24;11(4):273. doi: 10.1038/s41419-020-2478-0

Fig. 4. Knockdown of DDX11 suppresses cell proliferation, migration and invasion, and induces apoptosis of HCC cells in vitro.

Fig. 4

a DDX11 protein and mRNA expression in HCC cell lines or in normal liver cell line were analyzed by western blot and qPCR. b Western blot and qPCR analysis of DDX11 expression in HepG2 or SMMC7721 cells after transfection of sh-NC or sh-DDX11. HepG2 or SMMC7721 cells were transfected with sh-NC or sh-DDX11. c–e Cell proliferation and DNA synthesis were analyzed by CCK-8 assay, colony formation assay and EdU immunofluorescence staining respectively. f Cell invasion ability was determined by transwell assay. g, h Cell apoptosis and cell cycle analysis of HepG2 or SMMC7721 cells transfected with sh-NC or sh-DDX11 were analyzed by flow cytometer using Annexin V/PI or PI staining. i Western blot analysis of cell apoptosis and cell cycle related proteins in HepG2 or SMMC7721 cells with or without DDX11 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001.