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. 2020 Apr 24;11(4):273. doi: 10.1038/s41419-020-2478-0

Fig. 7. E2F1 activates DDX11 transcription in HCC cells.

Fig. 7

a E2F1 was speculated as an upstream factor of DDX11 based on differential E2F1expression analysis between normal tissues and tumor tissues (a left panel) and Pearson analysis of the correlation between DDX11 and E2F1 expression in TCGA-LIHC cohort (a right panel). b, c qPCR and western blot (d) analysis of the E2F1 and DDX11 expression in HepG2 or SMMC7721 cells transfected with E2F1 plasmid and E2F1 siRNA, respectively. e The sequence logo of a potential E2F1 binding site in JASPAR and a diagram of mutant sites in the DDX11 sequence. f, g Dual-luciferase reporter assay was performed by co-transfection of the DDX11 promoter fragment (WT or MUT pGL3-DDX11) and an E2F1-overexpression construct into HEK293 cells. h ChIP-seq analysis of E2F1 binding in DDX11 gene locus based on the data from GSE95305. i, j ChIP assay was performed using E2F1 antibody to determine the E2F1 binding to the DDX11 gene promoter. *p < 0.05, **p < 0.01, ***p < 0.001.