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. 2020 Apr 24;11(4):278. doi: 10.1038/s41419-020-2485-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Overexpression efficacy of ALDOA in sh-KCNQ1OT1 OS cells (U-2OS and 143B) was detected by western blotting. b Overexpression of ALDOA partly reversed the suppressed effects of KCNQ1OT1-knockdown on the colony formation capability of U-2OS and 143B cells, values are means ± SD, **p < 0.01 (Student’s t-test). c and d Overexpression of ALDOA partly reversed the induced effect of KCNQ1OT1-knockdown on the apoptosis of OS cells (U-2OS and 143B). Values are means ± SD, *p < 0.05, **p < 0.01 (Student’s t-test). e Morphologic characteristics of the xenograft tumors from U-2OS/sh-Control group, U-2OS/sh-KCNQ1OT1 group and U-2OS/sh-KCNQ1OT1 + ALDOA group (n = 5). Scale bars = 1 cm. f Overexpression of ALDOA partly rescued the inhibitory effects of KCNQ1OT1-knockdown on the growth rate of U-2OS cells in vivo. The volumes of tumors were measured every 5 days; values are means ± SD, *p < 0.05 (Student’s t-test). g The ECAR in OS cells (MNNG-HOS and U-2OS) in different groups (sh-Control, sh-KCNQ1OT1, and sh-KCNQ1OT1 + ov-ALDOA) were determined. Values are means ± SD. h Quantification of the glycolytic capacity from Fig. 3g, **p < 0.01, ***p < 0.001 (Student’s t-test). i The OCR in OS cells (U-2OS and 143B) in different groups (sh-Control, sh-KCNQ1OT1, and sh-KCNQ1OT1 + ov-ALDOA) were determined. Values are means ± SD. j Quantification of maximal respiration from the Fig. 3i, **p < 0.01, ***p < 0.001 (Student’s t-test).