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. 2020 Apr 24;11(4):278. doi: 10.1038/s41419-020-2485-1

Fig. 6. MiR-34c-5p inhibition partly rescued the KCNQ1OT1 knockdown effect in OS cells.

Fig. 6

a Western blot showed the ALDOA expression in U-2OS and 143B cells transfected with miR-34c-5pinhibitor, sh-KCNQ1OT1, or negative control. b miR-34c-5-knockdown partly reversed the inhibitory effects of KCNQ1OT1-knockdown on the colony formation properties of U-2OS and 143B cells and silenced miR-34c-5 in wide type OS cells (U-2OS and 143B) also promoted their proliferation, values are means ± SD, *p < 0.05, **p < 0.01 (Student’s t-test). c and d miR-34c-5-knockdown partly reversed the induce effect of KCNQ1OT1-knockdown on the apoptosis of OS cells (U-2OS and 143B). Knockdown of miR-34c-5 inhibited apoptosis of wide OS cells. Values are means ± SD, *p < 0.05, **p < 0.01 (Student’s t-test). e Altered levels of ECAR in the OS cells (U-2OS or 143B) in different groups (sh-Control, sh-KCNQ1OT1, sh-KCNQ1OT1 + anti-miR-34c-5p and anti-miR-34c-5p). Values are means ± SD. f Altered levels of OCR in the OS cells (U-2OS or 143B) in different groups (sh-Control, sh-KCNQ1OT1, sh-KCNQ1OT1 + anti-miR-34c-5p and anti-miR-34c-5p). Values are means ± SD. g Morphologic characteristics of xenograft tumors from U-2OS/sh-Control group, U-2OS/sh-KCNQ1OT1 group and U-2OS/sh-KCNQ1OT1 + anti-miR-34c-5p group (n = 5). Scale bars = 1 cm. h Anti-miR-34c-5p partly rescued the inhibitory effects of KCNQ1OT1-knockdown on the growth rate of U-2OS cells in vivo. The volumes of tumors were measured every 5 days; values are means ± SD, *p < 0.05, **p < 0.01 (Student’s t-test).