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. 2020 Apr 23;11(4):265. doi: 10.1038/s41419-020-2432-1

Fig. 1. The growth inhibition of RAGE-overexpressed A549 cells.

Fig. 1

An expression vector containing human RAGE open reading frame (pUNO1-hAGER, InvivoGene) or empty-vector (pUNO1-mcs) was transfected into A549 cells following by continuous screening for 8 weeks. a Western blotting was performed to evaluate the protein levels of RAGE in different subclones. β-actin served as the internal control. Two subclones were selected and renamed as RAGElow and RAGEhigh for future examination. b The intracellular distributions of RAGE (green signal) in A549 stable cell lines were monitored by immunocytochemistry, and DAPI was the marker of nucleus (blue signal). c 5 × 103 cells/well were seeded into 24-well culture plates and cultured with complete medium. The cell number was counting every 24 h and the cell growth of different subclones of A549 cells was shown. d Cell cycle distribution of A549 stable cell lines was analyzed by flow cytometry after propidium iodide (PI) staining. The quantitative data from three repeated samples was also provided. Parental cell line was used as the control group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with control group.