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. 2020 Apr 24;10:6935. doi: 10.1038/s41598-020-63978-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Confocal micrograph of a single optical section 10 µm from the surface of the block showing prehypertrophic chondrocytes with fluorescent labelling in form of a reticular network around the nucleus. (B,C) are transmission electron micrographs of prehypertrophic chondrocytes with rough endoplasmic reticulum, scattered mitochondria and some lipid droplets. A correspondence is found between the fluorescent labelling and the Intracellular organelles viewed with the electron microscopy. (D–F) are excitation-emission maps of a positive sample (F+/E+) and two control samples for background fluorescence: F+/E−, fixed non-eosin stained; F−/E−, non-fixed and non-eosin stained. The map of F+/E+ shows a broad range of effective excitation wavelengths and peaks in intensity at excitation/emission wavelengths of 531/556 nm. The map of F+/E− shows a significant decrease of fluorescence and the excitation/emission peak is found at 649/674 nm. Native autofluorescence due to the intrinsic components of the chondrocytes (F−/E−) was low. Color scale bar represents fluorescence intensity in arbitrary units.