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. 2020 Apr 23;10:6878. doi: 10.1038/s41598-020-63336-6

Figure 5.

Figure 5

Effects of Gap27 on Cx43 protein expression in AV-Shunt rats. (a) Representative immunoblot of Cx43 expression in the rat left ventricle. (b) Densitometry analysis of Cx43 total relative expression. GADPH expression was analysed as the loading control. (c) Representative immunofluorescence of left ventricle labelled with anti-Cx43 antibody (green) and β-actin (pink) from one ShamScrrat, one Shamgap27 rat, one AV-ShuntScr rat and one AV-ShuntGap27 rat. Scale bar 25 µm. Note that in AV-Shuntscr animals the patterns of Cx43 staining is lateralized compared to Shamscr animals. Values are shown as mean ± SEM. Data were analysed with Kruskal-Wallis test followed by Dunn post hoc analysis. *p < 0.05 vs. ShamScr. n = 8 per group.