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. 2020 Apr 24;11(4):272. doi: 10.1038/s41419-020-2457-5

Fig. 3. The effect of FADS1 depletion on the proliferation, migration and invasion of laryngeal cancer cell lines (TU212 and HN8).

Fig. 3

a CCK-8 assay was conducted to determine the proliferative ability of the indicated LSCC cells (Con, KD). b Colony formation assay was conducted in soft agar and an average colony number in multiple fields was counted in FADS1-KD and Con cells. c Flow cytometry analysis of cell apoptosis of the indicated laryngeal cancer cells (Con, KD) using propidium iodide/annexin V double staining. d Representative images of the cell cycle analysis of the indicated laryngeal cancer cells (Con, KD). e The representative images of the wound-healing assay of the indicated laryngeal cancer cells (Con, KD). Migration was monitored by light microscopy at 0 h and 24 h with ×100 magnification. f Transwell assay was conducted to detect the migratory and invasive ability of the indicated laryngeal cancer cells (Con, KD) with ×200 magnification. Quantitative analysis was shown on the right side of each representative figure. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test. All experiments were performed in triplicates and data are presented as mean ± SEM.