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. 2020 Apr 24;11(4):272. doi: 10.1038/s41419-020-2457-5

Fig. 4. The effect of FADS1 overexpression on proliferation, migration and invasion of laryngeal cancer cell lines (TU212 and HN8).

Fig. 4

a Growth curves of FADS1-OE and NC cells were determined by CCK-8 assay. b Colony formation assay was conducted and an average colony number in multiple fields was counted in FADS1-OE and NC cells. c The representative figure of the wound-healing assay of the indicated laryngeal cancer cells (OE, NC). Migration was monitored by light microscopy at 0 h and 24 h with ×100 magnification. d Transwell assay was conducted to detect the migratory and invasive ability of the indicated laryngeal cancer cells (OE, NC) with ×200 magnification. Each representative image was shown with quantitative analysis on the right side. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test. All experiments were performed in triplicates and all data are represented as mean ± SEM.