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. 2020 Apr 24;3:193. doi: 10.1038/s42003-020-0933-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Venn diagram depicting the number of targets identified in two independent RNA-seq experiments. FOXQ1 is a direct HuR target, which is also one of the top mRNAs decreased by KH-3 treatment. b Protein expression levels of HuR, FOXQ1 and E-cadherin in HMEC and a panel of TNBC cell lines. c, d Pull-down analysis of KH-3 disrupting ARE^FOXQ1 oligo binding to endogenous HuR in MDA-MB-231 and SUM159 cells. c Representative western blot result from one experiment. d Quantified relative HuR expression. Values are mean ± SD from n = 3 independent experiments (*P < 0.05, ***P < 0.001, one-way ANOVA). e, f RNP IP analysis of HuR bound FOXQ1 mRNA affected by KH-3 in MDA-MB-231 (e) and SUM159 (f) cells. Values are mean ± SD from three independent experiments (**P < 0.01, ***P < 0.001, one-way ANOVA). g, h Relative FOXQ1 mRNA levels in MDA-MB-231 (g) and SUM159 (h) cells treated with DMSO, KH-3 or KH-3B at the indicated time points. Values are mean ± SD from n = 3 independent experiments (***P < 0.001, two-way ANOVA). i, j FOXQ1 3′-UTR luciferase reporter assay in MDA-MB-231 (i) and SUM159 (j) cells treated by DMSO, KH-3B or KH-3. Values are mean ± SD from n = 3 independent experiments (***P < 0.001, two-way ANOVA).