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. 2020 Apr 24;11:2022. doi: 10.1038/s41467-020-15962-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Mbnl1, Mbnl2 and Mbnl3 gene expression levels during thymus organogenesis and in the developed gland. RNA-seq was performed at embryonic (E) days: 12.5 (n = 3), 13.5 (n = 3), 14.5 (n = 2), 15.5 (n = 2), 16.5 (n = 3), 17.5 (n = 2), 18.5 (n = 2) and postnatal days 0 (P0; n = 2) and 63 (P63; n = 3). Points connected by lines show mean expression ± standard deviation (SD). Data from embryonic and P0 thymi were obtained from GSE107910. b Survival curves for 129-Mbnl1 knockout (KO) (n = 124, female/male ratio is 49/75), B6-Mbnl1 KO (n = 33, 16/17), 129-wild type (WT) (n = 21, 7/14) and B6-WT (n = 21, 10/11). Significant differences between survival distributions were determined by Mantel–Cox (log-rank) test: *** P < 0.001; **** P < 0.0001. Median survivals are 22 and 37 weeks of age for 129-Mbnl1 KO and B6-Mbnl1 KO, respectively. There are no significant differences between females and males. c Gene expression changes in 129-Mbnl1 KO thymus. Pie chart represents the proportion of significantly altered genes (blue) to all detected genes (gray) in 129-Mbnl1 KO (n = 3) compared to WT (n = 3) RNA-seq samples. d Scatter plot represents 44 downregulated genes encoding immunoglobulin heavy (Ighv; 20 genes), kappa (Igkv; 17 genes) and other (7 genes) chains. Each dot represents a significantly changed gene by DESeq2 analysis. The most affected gene for each group is indicated. e Reduction of the TCR repertoire detected by Mbnl1 KO RNA-seq. Bar graph shows number of unique Tcrb and Tcra sequences normalized to the unique mapped read count ± SD. Significant difference was determined by two-tailed t-test: * P = 0.027. f Tcrb (n = 3) and Tcra (n = 3) repertoire diversity reflected by Shannon’s equitability where 1 equals complete equivalency. g Pie charts demonstrate representative Tcrb and Tcra clonotype frequencies in 129-Mbnl1 KO and WT thymic RNA-seq. Gray areas represent unique clones from the total clone count. Source data are provided as a Supplementary Data 1 file.