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. 2020 Apr 24;11:2022. doi: 10.1038/s41467-020-15962-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative photos of male WT and 129-Mbnl1 KO thymi at 4, 12, and 22 weeks of age. The normal process of thymic involution that occurs in WT is disrupted in 129-Mbnl1 KO at 22 weeks. b Male thymic wet weight normalized to body weight is significantly increased in 129-Mbnl1 KO (n = 10) compared to WT (n = 6) at 19–27 weeks. There is no significant difference between 4 and 13 weeks (WT = 50, KO = 18). Bar graph shows mean ± SD. Significant difference was determined by the Mann-Whitney test: **P = 0.003. c Hematoxylin and eosin (H&E) stain of WT and enlarged 129-Mbnl1 KO thymi. d Increased number of viable thymocytes in enlarged 129-Mbnl KO (n = 3) compared to WT (n = 6) thymi by ~22 (20± 2) weeks. Bar graph shows mean ± SD. Significant difference was determined by the Mann–Whitney test: *P = 0.024. e Representative flow cytometry analysis of CD4 and CD8 expression in ~22 weeks WT and 129-Mbnl1 KO thymocytes. f Thymocyte composition at 4 (WT = 3; KO = 3), 8 (WT = 8; KO = 6), and ~22 (WT = 4; KO = 3) weeks. CD4⁺, CD8⁺, CD4⁺CD8⁺ double positive (DP) and CD4^-CD8^- double negative (DN) thymocytes were analyzed by flow cytometry. Graph represents mean KO–WT subpopulations ± SD. Significant difference was determined by the Mann–Whitney test: # P = 0.057, **P = 0.007, ***P = 0 .0007. g Representative flow cytometry Forward Scatter (FSC) parameter plots showing different relative thymocyte CD8⁺ cell sizes in 129-Mbnl1 KO. Source data are provided as a Supplementary Data 1 file.