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. 2020 Apr 24;11:2022. doi: 10.1038/s41467-020-15962-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Alternative splicing (AS) changes in 129-Mbnl1 KO thymus. Pie chart represents the proportion of significantly altered AS events (blue) to all detected AS events (gray) in the 129-Mbnl1 KO (n = 3) compared to WT (n = 3). b Number of significantly changed AS events categorized as skipped exons (SE), alternative 5′ and 3′ splice sites (A5SS and A3SS), mutually exclusive exons (MXE) and retained introns (RI). c Wide range of splicing changes. Box plot of AS event | ΔPSI | values. PSI—percent spliced in. Center line represents the median, and the box extends from the 25th to 75th percentiles. Whiskers show 1–99 percentile. d Heat map illustrates selected 27 AS events changed in Mbnl1 KO thymus (compare to Fig. 5e). ** FDR < 0.01, **** FDR < 0.0001. e Sashimi plot of Mbnl1 KO (n = 3) and WT (n = 3) RNA-seq samples for Lef1 E5-E6-E7. Mean PSI values are provided below the plots. f Lef1 E6 exclusion in 129-Mbnl1 KO (n = 3) compared to WT (n = 3) thymi detected by RT-PCR. Bar graph shows mean PSI ± SD. Significant difference was determined by the two-tailed t-test: **** P < 0.0001. g Representative gel of Lef1 E6 RT-PCR assay from the different Mbnl1 KO strains as well as Mbnl2 and Mbnl3 KOs. PSI values are provided below each gel lane. h Genome browser view of RNA-seq generated from C2C12 cells with the compound Mbnl1 and Mbnl2 knockdown by shRNA. The cluster of MBNL1 CLIP-seq reads indicates MBNL1 binding sites in C2C12. i MBNL1 (n = 4) as well as compound MBNL1 and MBNL2 (n = 4) knockdown causes LEF1 E6 exclusion in human T cells (Jurkat). Bar graph shows mean PSI ± SD. Significant difference was determined by Dunnett’s multiple comparison test: **** adjusted P value (P_adj) < 0.0001. Source data are provided as the Supplementary Data 1 and Source Data files.